Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay

Figure 2

Results of RT-PCR. Exponentially growing HEK293T cells were treated with 0–200 ng/mL Wnt3a for 24, 48, or 72 h. After these time points total RNA was isolated and semiquantitative RT-PCR was performed using CCND1, MYC, and, as loading control, GAPDH specific primers. For quantification, the expression was normalized to the level of GAPDH expression and the untreated control was set to 100%.