Pulling a Ligase out of a “HAT”: pCAF Mediates Ubiquitination of the Class II Transactivator

Figure 1

CIITA associates with the E3 ligase pCAF. Coimmunoprecipitation of CIITA and pCAF. COS cells were cotransfected with Myc-CIITA and Flag-pCAF. Cells were harvested, lysed, precleared, and immunoprecipitated with anti-Flag and anti-Mouse IgG. Western blots were performed and immunoprecipitated samples were immunoblotted using anti-Myc antibodies. Lysate controls demonstrate expressions of Myc-CIITA and Flag-pCAF. Data shown are cropped images from one immunoprecipitation gel and one lysate gel and are representative of three individual experiments.