Pulling a Ligase out of a “HAT”: pCAF Mediates Ubiquitination of the Class II Transactivator

Figure 2

The pCAF E3 ligase domain is necessary for enhanced CIITA transactivity. CIITA transactivation increases in the presence of expressed WT pCAF. Reporter Assay. COS cells were transfected as indicated with the MHC II HLA-DR Luc reporter construct, Renilla, CIITA, pCAF, and Δ E3 pCAF. Luciferase assays were performed in triplicate in three independent experiments; data are presented as fold increase in luciferase activity. Results are standardized to Renilla values and represent the mean ± SD, < 0.01.