Pulling a Ligase out of a “HAT”: pCAF Mediates Ubiquitination of the Class II Transactivator

Figure 5

Acetylation null CIITA mutants coimmunoprecipitate with pCAF. Coimmunoprecipitation of acetylation null CIITA mutants with pCAF. COS cells were cotransfected with Myc-CIITA, K141R CIITA, K144R CIITA or K141/144R CIITA, and Flag-pCAF. Cells were harvested, lysed, precleared, and immunoprecipitated with anti-Myc or Mouse IgG antibodies as indicated. Western blots were performed and immunoprecipitated samples were immunoblotted using anti-Myc antibodies. Lysate controls demonstrate expression of Myc-CIITA and acetylation null mutants and Flag-pCAF (bottom two panels). Data shown are cropped images from one immunoprecipitation gel and one lysate gel and are representative of 3 individual experiments.