Pulling a Ligase out of a “HAT”: pCAF Mediates Ubiquitination of the Class II Transactivator

Figure 7

pCAF ubiquitinates CIITA in vitro. (a) Ubiquitination of CIITA by pCAF in vitro. Reaction components are as indicated and were performed as described in Material and Methods. Ubiquitination was detected with anti-Flag antibody (top panel); pCAF was detected using anti-pCAF antibody (bottom panel). (b) Ubiquitinated CIITA was further detected with anti-GST antibody. (c) Positive control demonstrating pCAF’s ability to facilitate ubiquitination in vitro on Hdm2.

(a)

(b)

(c)