Odontoblast-Like Cells Differentiated from Dental Pulp Stem Cells Retain Their Phenotype after Subcultivation

Figure 5

Relative gene expression quantitation during differentiation. Specific odontoblast and mineralizing transcripts were quantitated in differentiating OLC (7, 14, and 21 days) and in both fresh and thawed EXP-21 cells by real-time PCR considering a value of 1 in hDPSC. Data are shown as mean ± SD of three independent experiments in duplicate (). Runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osterix (OSX), type I-collagen (COL-I), dentin sialophosphoprotein (DSPP), dentin matrix protein-1 (DMP-1), osteopontin (OPN).