Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis

Figure 2

Induction of cell death in A549 lung cancer cells with downregulation of RUNX2. A549 cells were treated with doxorubicin or vehicle for 24 h and 48 h after pretreatment with lentiviral particles coding for sh-RNA against RUNX2. (a). A representative contour plot of A549 cells treated with DOXO and vehicle for 24 h, pretreated with sh-RUNX2 and sh-Ctrl, and labeled with Annexin V-FICT and PI is shown. (b). A549 cells were treated with DOXO and vehicle for 48 h, pretreated with sh-RUNX2 and sh-Ctrl, and labeled with Annexin V-FICT and PI. Right panels show the percentages of dead cells are shown in bars of (three independent experiments) , , .

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