Regulatory Role of the RUNX2 Transcription Factor in Lung Cancer Apoptosis

Figure 3

Knockdown of RUNX2 and proapoptotic gene expression levels: BAD and BAX. A549 cells were infected with lentiviral particles coding for sh-RNAs against RUNX2. BAD (a) and BAX (d) mRNA levels were quantified by RT-qPCR 48 h after infection. Statistical analyses were performed with respect to cells infected with the virus generated with the pLKO.1 empty vector (sh-Ctrl.). (three independent experiments). Protein levels were analyzed with immunofluorescence assays. BAD protein expression mitochondrial pattern (b). BAX protein expression with nuclear localization (e). Total cell fluorescence (CTCF) of protein was evaluated () using the software ImageJ 1.52p (c, f).

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