Consequences of Neutralization on the Proliferation and Cytoskeletal Organization of Chondrocytes on Chitosan-Based Matrices

Figure 1

FTIR spectra for nonneutralized films showing the peaks at 1560 cm⁻¹ and 1400 cm⁻¹ corresponding to the asymmetric and symmetric carboxylate ion stretching [48] characteristic of the presence of acetate ion in the chitosan acetate salt. FTIR spectra of chitosan films that were neutralized with varying concentrations of NaOH show the amide I (C=O stretching) and amide II (N–H bending) peaks at 1656 cm⁻¹ and 1546 cm⁻¹, respectively, characteristic of chitosans not completely deacetylated. Also they show the presence of a peak at 1591 cm⁻¹ which corresponds to the N–H stretching of the NH₂ (amine) group.