Consequences of Neutralization on the Proliferation and Cytoskeletal Organization of Chondrocytes on Chitosan-Based Matrices
Figure 3
SDS-PAGE analysis of surface adsorbed proteins. Scaffolds that were subjected to the indicated pretreatments were exposed to serum and the surface adsorbed proteins were eluted and analyzed by gel electrophoresis. Typically, 3 to 4 scaffolds were treated as independent experiments. (a) SDS-PAGE of proteins eluates. PL: protein ladder. Lane 1: 0.1 M NaOH concentration for 10 min., Lane 2: 0.1 M NaOH concentration for 30 min, Lane 3: 0.25 M NaOH concentration for 10 min., Lane 4: 0.25 M NaOH concentration for 30 min., Lane 5: 0.5 M NaOH concentration for 10 min., and Lane 6: 0.5 M NaOH concentration for 30 min. (b) Semiquantitative evaluation of the gel image in (a) with ImageJ software.
