Consequences of Neutralization on the Proliferation and Cytoskeletal Organization of Chondrocytes on Chitosan-Based Matrices
Figure 6
Visualization of actin and vinculin distribution by confocal microscopy. Cells on either glass slides or chitosan films were stained for actin or vinculin as detailed in the methods section. Panels (a), (b), and (c) shows the distribution of actin/vinculin on glass slides. Panels (d), (e), (f), (g), (h), and (i) show the distribution of actin/vinculin on chitosan films. Panel (d) shows chitosan films neutralized with 0.1 M NaOH for 10 minutes, panel (e) shows chitosan films neutralized with 0.25 M NaOH for 10 minutes, and panel (f) shows chitosan scaffolds neutralized with 0.5 M NaOH for 10 minutes, panel (g) shows chitosan films neutralized with 0.1 M NaOH for 30 minutes, panel (h) shows chitosan films neutralized with 0.25 M NaOH for 30 minutes, and panel (i) shows chitosan films neutralized with 0.5 M NaOH for 30 minutes. Similar distributions were noted at different incubation times evaluated. Scale bar: 10 μm.
