Research Article
A New Rapid and Sensitive Spectrophotometric Method for Determination of a Biopolymer Chitosan
Table 1
Stability of color complex by the proposed method.
| Time after color development (min) |
Absorbance (555 nm) with the following amount of chitosan (μg/mL) | 5 | 10 | 25 |
| 5 | 0.086 | 0.151 | 0.410 | 10 | 0.086 | 0.152 | 0.412 | 15 | 0.091 | 0.153 | 0.415 | 20 | 0.095 | 0.154 | 0.411 | 25 | 0.098 | 0.152 | 0.410 | 30 | 0.098 | 0.152 | 0.411 | 35 | 0.101 | 0.154 | 0.412 | 40 | 0.100 | 0.152 | 0.411 | 45 | 0.100 | 0.154 | 0.409 | 50 | 0.102 | 0.157 | 0.409 | 55 | 0.097 | 0.155 | 0.404 | 60 | 0.100 | 0.157 | 0.405 | 90 | 0.092 | 0.153 | 0.402 | 120 | 0.092 | 0.153 | 0.409 | 150 | 0.092 | 0.150 | 0.407 | 180 | 0.092 | 0.150 | 0.414 | 1080 (18 h) | 0.046 | 0.080 | 0.231 |
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The values are means of four replicates.
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