A New Rapid and Sensitive Spectrophotometric Method for Determination of a Biopolymer Chitosan

Table 2

Determination of chitosan in pharmaceutical formulation by the proposed method.

Sample	Chitosan content declared in label (mg)	Sample taken (μL)	Chitosan calculated (mg)	Chitosan found (mg)	% of chitosan in formulation ± SE	Recovery (%)^a  ±  SE
		20	0.029	0.022	73.82±1.54	96.67±1.75
		40	0.059	0.044	74.86±1.87	97.28±0.88
Chitocal capsule	500 mg	60	0.088	0.067	75.52±1.84	97.89±0.56
		80	0.118	0.089	75.59±2.19	98.11±1.15
		100	0.147	0.119	80.81±2.51	98.46±0.82
Mean ± SE					76.11±1.55	97.68±0.32

^a Recovery (%) values were calculated from the amount of chitosan that had been determined after addition of its different amounts to a fixed amount of dosage form (capsule). The data are average of three determintaions.