Effect of Additives and Process Variables on Enzymatic Hydrolysis of Macauba Kernel Oil (Acrocomia aculeata)

Table 2

Effect of additives in the hydrolysis yield of macauba kernel oil at 55°C, 400 rpm, buffer to oil mass ratio of 1 : 2, buffer pH of 8.0, catalyst percentage of 5%, and 6 hours of reaction.

	Additives	Percentage (%)	Yield (wt%)
	Without	0	67.30±0.19^A
Surfactant; (in relation to mass substrate)	Triton X-100	5	60.00±0.09^B;a,e
		10	45.89±0.08^C;b
		15	43.88±0.74^D;c
	Tween 80	5	57.01±0.19^E;d
		10	60.58±0.03^F;e
		15	58.33±0.00^G;f
Salts	CuCl2	0.01 M in buffer	58.63±0.30^H;a′,d′
	CaCl2		61.77±0.04^I;b′,c′,d′
	KCl		62.65±0.26^J;A;c′,d′,f′
	NH4Cl		61.06±0.09^K;d′
	NaCl		68.07±0.05^L,A;e′
	MgCl2		65.07±0.11^M,A;f′

Means followed by same uppercase letters (compared with the sample without additives) and lowercase letters (for same additives) did not differ statistically (𝑃>0.05).