Research Article
Optimization of Process Parameters by Statistical Experimental Designs for the Production of Naringinase Enzyme by Marine Fungi
Table 4
Levels of five different factors, applied in each of 16 trials, and the obtained results.
| | Trial number | Factor 1
A: Initial pH | Factor 2
B: Moisture content (mL) | Factor 3
C: Weight of grape fruit (%) | Factor 4
D: Weight of NaNO3 (%) | Factor 5
E: Weight of waste (g) | Response enzyme
activity (U/mL) |
| | 1 | 1 | 4 | 4 | 4 | 4 | 0.00 | | 2 | 2 | 4 | 3 | 2 | 1 | 5.46 | | 3 | 2 | 1 | 2 | 3 | 4 | 0.00 | | 4 | 4 | 2 | 3 | 1 | 4 | 6.76 | | 5 | 3 | 3 | 1 | 2 | 4 | 13.00 | | 6 | 1 | 1 | 1 | 1 | 1 | 6.50 | | 7 | 1 | 2 | 2 | 2 | 2 | 0.00 | | 8 | 3 | 4 | 2 | 1 | 3 | 7.54 | | 9 | 3 | 2 | 4 | 3 | 1 | 6.50 | | 10 | 4 | 4 | 1 | 3 | 2 | 5.98 | | 11 | 2 | 2 | 1 | 4 | 3 | 6.76 | | 12 | 3 | 1 | 3 | 4 | 2 | 8.06 | | 13 | 1 | 3 | 3 | 3 | 3 | 8.32 | | 14 | 2 | 3 | 4 | 1 | 2 | 0.00 | | 15 | 4 | 1 | 4 | 2 | 3 | 0.00 | | 16 | 4 | 3 | 2 | 4 | 1 | 5.72 |
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The numbers under factors refer to their levels; for description of levels, refer to Table 3.
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