Research Article
Some Investigations on Protease Enzyme Production Kinetics Using Bacillus licheniformis BBRC 100053 and Effects of Inhibitors on Protease Activity
Table 2
The absorbance of the samples with different PMSF inhibitor concentrations.
| OD (280 nm) | | Time (min) | | | | |
| 0.001 | 1 | 0.637 | 0.643 | 0.616 | 0.750 | 2 | 0.652 | 0.658 | 0.675 | 0.812 | 3 | 0.713 | 0.715 | 0.694 | 0.834 | 4 | 0.739 | 0.760 | 0.767 | 0.924 | 5 | 0.785 | 0.808 | 0.808 | 0.974 |
| 0.002 | 1 | 0.477 | 0.483 | 0.487 | 0.533 | 2 | 0.514 | 0.525 | 0.533 | 0.540 | 3 | 0.540 | 0.543 | 0.571 | 0.579 | 4 | 0.578 | 0.600 | 0.619 | 0.675 | 5 | 0.608 | 0.634 | 0.655 | — |
| 0.0075 | 1 | 0.371 | 0.374 | 0.375 | 0.379 | 2 | 0.392 | 0.399 | 0.405 | 0.410 | 3 | 0.407 | 0.416 | 0.424 | 0.430 | 4 | 0.433 | 0.445 | 0.456 | 0.467 | 5 | — | — | 0.477 | — |
| 0.025 | 1 | 0.256 | 0.258 | 0.259 | 0.259 | 2 | 0.267 | 0.272 | 0.273 | 0.277 | 3 | 0.272 | 0.276 | 0.280 | 0.284 | 4 | 0.287 | 0.291 | 0.297 | 0.302 | | 5 | — | — | — | — |
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