Some Investigations on Protease Enzyme Production Kinetics Using Bacillus licheniformis BBRC 100053 and Effects of Inhibitors on Protease Activity

Table 3

The absorbance of the samples with different DFP inhibitor concentrations.

OD (280 nm)
𝑋=(𝑉𝑖/𝑉𝑜)	Time (min)	𝑆1=0.75%	𝑆2=1%	𝑆3=1.25%	𝑆4=1.5%
0.0004	1	1.065	1.074	1.079	1.084
	2	1.111	1.124	1.139	1.148
	3	1.151	1.179	1.191	1.204
	4	1.198	1.224	1.254	1.171
	5	—	1.281	1.308	—
0.001	1	0.669	0.676	0.680	0.685
	2	0.711	0.724	0.735	0.743
	3	0.745	0.864	0.781	0.792
	4	0.789	0.816	0.836	0.855
	5	—	—	—	—
0.005	1	0.374	0.378	0.381	0.377
	2	0.4	0.41	0.414	0.414
	3	0.419	0.419	0.439	0.439
	4	0.448	0.465	0.476	0.482
	5	—	0.491	—	0.515
0.01	1	0.234	0.237	0.238	0.239
	2	0.251	0.257	0.26	0.265
	3	0.26	0.267	0.274	0.28
	4	0.26	0.267	0.299	0.308
	5	—	—	—	—