Microwave-Assisted Method for Simultaneous Hydrolysis and Extraction for Preparation of Geniposidic Acid from Eucommia ulmoides Bark Using Basic Imidazolide Ionic Liquid
Table 1
Experimental design matrix to screen for variables that determine the yield of geniposidic acid and ANOVA results.
(a)
Run
BBD experiments
1
15 (0)
15 (−1)
540 (+1)
2.98
2
15 (0)
15 (−1)
230 (−1)
2.58
3
15 (0)
25 (+1)
230 (−1)
2.87
4
20 (+1)
25 (+1)
385 (0)
3.30
5
10 (−1)
25 (+1)
385 (0)
3.02
6
20 (+1)
20 (0)
230 (−1)
2.72
7
20 (+1)
15 (−1)
385 (0)
3.48
8
20 (+1)
20 (0)
540 (+1)
2.85
9
10 (−1)
15 (−1)
385 (0)
2.82
10
15 (0)
20 (0)
385 (0)
3.30
11
10 (−1)
20 (0)
540 (+1)
2.63
12
15 (0)
20 (0)
385 (0)
3.25
13
15 (0)
20 (0)
385 (0)
3.12
14
15 (0)
20 (0)
385 (0)
3.15
15
15 (0)
20 (0)
385 (0)
3.22
16
15 (0)
25 (+1)
540 (+1)
2.68
17
10 (−1)
20 (0)
230 (−1)
2.34
(b)
ANOVA
Source
Sum of squares
Degree of freedom
Mean square
-value
value
Model
1.49
9
0.17
28.49
0.0001
0.30
1
0.30
51.43
0.0002
1
0.9810
0.050
1
0.050
8.68
0.0215
0.036
1
0.036
6.17
0.0420
1
1.33
0.2874
0.085
1
0.085
14.67
0.0065
0.040
1
0.040
6.85
0.0346
1
1.45
0.2673
0.95
1
0.95
163.18
<0.0001
Residual
0.041
7
Lack of fit
0.020
3
1.28
0.3942
Pure error
0.021
4
Cor total
1.53
16
Credibility analysis of the regression equations
Standard deviation
Mean
Coefficient of variation%
Adjusted
Adequacy precision
0.08
2.96
2.57
0.9734
0.9393
19.23
The results were obtained with Design-Expert 7.0 software. is the microwave irradiation time (min), is the liquid-solid ratio (mL/g), is the microwave irradiation power (W), and Y is the yield of geniposidic acid (μmol/g). Significant at .
