Analysis of Polymorphisms in the Lactotransferrin Gene Promoter and Dental Caries

Table 3

Reaction conditions for melting acquisition performed by LightScanner and LightCycler 480.

Program/cycles	Temperature
Preincubation/01	Initial heating: 95°C
Amplification/45	Denaturation: 95°C
	Annealing: 68°C
	Final Extension: 72°C
High-resolution melting/1	Heating: 95°C
	Hybridzation: 40°C
	Melting acquisition: 65°C to 95°C
Cooling/1	40°C