Peri-Implant Crestal Bone Loss: A Putative Mechanism
Figure 1
Expression of pro-inflammatory and osteoclastogenic genes with several LPS stimulation in human gingival fibroblasts. IL-1β, IL-6, TNF-α, RANL, RANKL, and GAPDH gene expression in hGF cultures with different types of LPS stimulation: E. coli, P. gingivalis, P. intermedia and without LPS for 2 hours. 1 μg/mL of LPS was used in the experiment. mRNA was isolated at 2 hours. RT-PCR was carried out at different cycles for each gene. Annealing temperature was 55 degrees for all genes.
