Peri-Implant Crestal Bone Loss: A Putative Mechanism
Figure 2
Expression of LPS receptors and their signaling factor’s genes with several LPS stimulation in human fibroblasts. TRL 2, TRL 4, CD14, and MD-2 gene expression in hGF cultures with different types of LPS stimulation: E. coli, P. gingivalis, P. intermedia, and without LPS for 2 hours. 1 μg/mL of LPS was used in the experiment. mRNA was isolated at 2 hours. RT-PCR was carried out at different annealing temperature that TRL2 and CD14 were 57 degrees, and TRL 4 and MD-2 were 54 degrees. PCR cycle was 30 cycles for all genes.
