Research Article

Peri-Implant Crestal Bone Loss: A Putative Mechanism

Figure 5

Expression of pro-inflammatory cytokine genes in human bone marrow cells. IL-1α, IL-1β, TNF-α, IL-6, and GAPDH gene expression in hBMCs cultures with or without LPS stimulation. LPS concentrations were 0 μg/mL, 0.1 μg/mL or 1 μg/mL. RNA was isolated at 24, 48, and 96 hours. RT-PCR was carried out at 30 cycles for GAPDH, IL-1α, IL-1β, and TNF-α, and 25 cycles for IL-6.
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