Peri-Implant Crestal Bone Loss: A Putative Mechanism

Figure 9

TRAP positive reactions were showed as level of absorbance in each group. The data were calculated by triplicate samples in each group. One day after mMO cells were seeded into 96 wells they were stimulated with E. coli, P. gingivalis, P. intermedia LPS for 3 days. 1 μg/mL of LPS was added into the cell culture medium individually. As positive control, we used 300 ng/mL GST-RANKL which was treated the same way with LPS groups. Negative control was not including RANKL and LPS.