Peri-Implant Crestal Bone Loss: A Putative Mechanism

Table 1

Specific primers for the reverse transcriptase polymerase chain reaction (RT-PCR) and the predicted sizes of the PCR products (bp).

(a) Specific primers for mouse

Specificity	Primer	Detection	Product size (bp)
IL-1alpha	Sense	TGCCACCAAAGAACAAAGTCG	599
	Antisense	CCCACTGAGGTAGGAAAGATGTAGC
IL-1beta	Sense	TCTTTGAAGTTGACGGACCCC	269
	Antisense	CCAGCAGGTTATCATCATCATCCC
TNF-alpha	Sense	ACCGTCAGCCGATTTGCTATC	288
	Antisense	TCAGAGTAAAGGGGTCAGAGTGGG
IL-6	Sense	GAGCCCACCAAGAACGATAG	229
	Antisense	TCCACGATTTCCCAGAGAAC

(b) Specific primers for rat

Specificity	Primer	Detection	Product size (bp)
IL-1alpha	Sense	AGGTTCCACGTTTCCTCCTT	233
	Antisense	GCCTCCAGGTCATCTTCAGT
IL-1beta	Sense	CAACAAAAATGCCTCGTGC	395
	Antisense	AAGTCAACTATGTCCCGAC
TNF-alpha	Sense	TACTGAACTTCGGGGTGATCG	292
	Antisense	CCTTGTCCCTTGAAGAGAACC
IL-6	Sense	TGTGCAATGGCAATTCTGAT	156
	Antisense	GGAACTCCAGAAGACCAGAGC

(c) Specific primers for human

Specificity	Primer	Detection	Product size (bp)
IL-1alpha	Sense	TTCATTGGCGTTTGAGTCAG	163
	Antisense	GGAGTGGGCCATAGCTTACA
IL-1beta	Sense	GCATCCAGCTACGAATCTCC	193
	Antisense	TCGTTATCCCATGTGTCGAA
TNF-alpha	Sense	ACAAGCCTGTAGCCCATGTT	265
	Antisense	TTGATGGCAGAGAGGAGGTT
IL-6	Sense	GCTATGAACTCCTTCTCCACAAGC	264
	Antisense	TTCTGCCAGTGCCTCTTTGC
RANK	Sense	ATGCGGTTTGCAGTTCTTCT	384
	Antisense	CGTAGGGACCACCTCCTACA
RANKL	Sense	AGAGCGCAGATGGATCCTAA	180
	Antisense	TTCCTTTTGCACAGCTCCTT
TRL 2	Sense	TTAGCAACAGTGACCTACAGAG	503
	Antisense	CAAATCAGTATCTCGCAGTTCC
TRL 4	Sense	TGGATACGTTTCCTTATAAG	507
	Antisense	GAAATGGAGGCACCCCTTC
CD 14	Sense	CAACTTCTCCGAACCTCAGC	271
	Antisense	TAGGTCCTCGAGCGTCAGTT
MD-2	Sense	GCACATTTTCTACATTCC	157
	Antisense	CACAGTCTCTCCCTTCAG

(d) Specific primers for House keeping gene

Specificity	Primer	Detection	Product size (bp)
GAPDH	Sense	ACCACAGTCCATGCCATCAC	452
	Antisense	TCCACCACCCTGTTGCTGTA