Peri-Implant Crestal Bone Loss: A Putative Mechanism
Table 3
ELISA results for hMO (a), hBMCs (b) and hGFs (c). For hMO, protein levels were assessed from the cell-free culture supernatants. hBMC and hGF protein levels were quantified from the cell lysate.
(a) Secretion of IL-1α and IL-1β by human macrophages after LPS stimulation
IL-1α
IL-1β
Stimulation period
LPS 0
LPS 1 μg/mL
LPS 0
LPS 1 μg/mL
24 hrs
0 pg/mL
470.80 ± 79.69 pg/mL
32 pg/mL
400 pg/mL
(b) Secretion of IL-1α, IL-1β, and TNF-by human bone marrow cells after LPS stimulation
IL-1α (pg/mL)
IL-1β (pg/mL)
TNF-α (pg/mL)
LPS (μg/mL)
LPS (μg/mL)
LPS (μg/mL)
Stimulation period
0
0.1
1
0
0.1
1
0
0.1
1
24 hrs
0
5.49 ± 1.21
5.11 ± 2.03
0
9.17 ± 4.57
16.15 ± 1.78
19.32 ± 10.03
52.95 ± 5.26
54.55 ± 3.25
48 hrs
0
0.34 ± 0.46
0
0
0
15.59 ± 1.05
22.27 ± 2.12
51.48 ± 2.33
40.00 ± 4.82
96 hrs
0
0
0
0
0
0
14.89 ± 2.44
42.39 ± 8.85
40.11 ± 11.06
(c) Secretion of IL-1α, IL-1β, and TNF-by human gingival fibroblasts after LPS stimulation
