Effect of S-PRG Eluate on Biofilm Formation and Enzyme Activity of Oral Bacteria

Figure 1

Adherence of Streptococcus mutans JCM 5705 in the presence and absence of PBS or S-PRG eluate. To evaluate the effect of S-PRG eluate on adherence, different percentages of S-PRG were substituted for culture medium (circle). For the control, phosphate-buffered saline was used instead of S-PRG (triangle). The plates were incubated at 37°C for 16 h under anaerobic conditions, stained with 0.25% safranin, and the amount of stain in an ethanol-solubilized sample quantified by measuring absorbance at 492 nm. Data are representative of three independent experiments and the bars indicate the standard deviation.