Porphyromonas gingivalis Outer Membrane Vesicles Mediate Coaggregation and Piggybacking of Treponema denticola and Lachnoanaerobaculum saburreum

Table 1

P. gingivalis outer membrane vesicle-induced coaggregation between T. denticola and L. saburreum.

Amount of outer membrane vesicles (𝜇g/mL)*	Coaggregation score^†
	T. denticola	L. saburreum	T. denticola + L. saburreum
0	0	0	0
25	0	0	0
50	0	0	1+
100	0	0	1+
250	0	0	1+
500	1	0	3+
500 (100°C/5 min)	0	0	0

*Final concentration in the assay mixture.
^†0: no visible coaggregates in the cell suspension; 1+: small uniform coaggregates in the cell suspension; 2+: definite coaggregates easily seen but suspension remained turbid; 3+: large coaggregates that settled rapidly, leaving some turbidity in the supernatant fluid; 4+: clear supernatant fluid and large coaggregates that settled immediately.