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International Journal of Dentistry
Volume 2013 (2013), Article ID 854765, 5 pages
http://dx.doi.org/10.1155/2013/854765
Research Article

Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria

1Department of Operative and Esthetic Dentistry, School of Dentistry, Ahvaz Jundishapour University of Medical Sciences, Ahvaz 613571-5775, Iran
2Department of Periodontology, School of Dentistry, Ahvaz Jundishapour University of Medical Sciences, Ahvaz 613571-5775, Iran
3Ahvaz Dental School Research Center, Ahvaz 613571-5775, Iran
4Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 61355-45, Iran
5Department of Pharmacognosy Clinical, Kerman School of Pharmacy, Kerman 76135-1614, Iran

Received 2 April 2013; Revised 21 May 2013; Accepted 22 May 2013

Academic Editor: Figen Seymen

Copyright © 2013 Faramarz Zakavi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. In this study antimicrobial effect of ethanolic and aqueous extracts of Juglans regia bark in Iran was evaluated on four different oral bacteria, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, and Staphylococcus aureus. Methods. Aqueous and ethanol extracts of Juglans regia bark were prepared by using disk diffusion technique and Minimal Inhibitory Concentration (MIC) methods. Tetracycline 30 μg and Erythromycin 15 μg were used as positive control and water as negative control in disk diffusion and MIC methods. Data were analyzed by ANOVA test. Results. The results showed that S. sanguis and S. mutans were the most sensitive and the most resistant bacteria against ethanolic and aqueous extracts, respectively. Ethanolic extract had significant antibacterial effect against all tested bacteria. Aqueous extract did not show antibacterial effect on S. mutans, in contrast to ethanolic extract. Aqueous extract had significantly antibacterial effect against Staphylococcus aureus, S. salivarius, and S. sanguis compared to control ( ), but it did not show effect on S. mutans when compared with Erythromycin. According to the obtained MIC values, ethanol extract of Juglans regia bark had the lowest rate. Conclusion. The results may provide the basis for using natural antimicrobial substance for oral hygiene prophylaxis purposes.