Prolactin and Male Fertility: The Long and Short Feedback Regulation
Figure 3
Release of PRL by PHC and PI of normal and castrated
rats incubated with or without testosterone for 72 hours. Upper panel. Pattern
of release of PRL by PHC and PI of castrated rats, incubated in absence () or presence () of testosterone (0.3 mM) for 72 hours. Values are mean ± SE and derived from 40 individual incubations
of PHC and 16 of PI, respectively. Lower panel. Pattern of release of PRL by
PHC and PI of normal rats incubated in absence () or presence () of testosterone (0.3 mM) for 72 hours. Values are mean ± SE and derived from 15 individual incubations
of PHC and 16 PI, respectively. , , denote significant difference in values at 48 hours
as compared to 24 hours, at 72 hours as compared to 48 hours, and at 72 hours
as compared to 24 hours, respectively. p, q, r denote significant difference in groups incubated
with or without testosterone at 24, 48, and 72 hours, respectively. *. Pituitary constructs were dissected out of normal
or 7-day castrated rats and incubated in DMEM with or without 0.3 mM
testosterone, at 37°C for 24–72 hrs. Prolactin was analysed in the spent medium by RIA
[54].
