Prolactin and Male Fertility: The Long and Short Feedback Regulation

Figure 5

Western blots of protamine 1 in epididymal sperm of sexually mature rats depicting the presence of 6 Kd protamine 1 bands in control and absence in drug-treated rats. Cp: Cyproterone acetate; C: Control; N: negative control. Basic proteins were extracted in HCl, differentially extracted in perchloric acid, analysed on 15% acid urea PAGE. Western blotting was done with 1Hup 1N monoclonal antibody provided by Dr. Rod Balhorn [61].