Comparison of the Pharmacological Effects of Paricalcitol and Doxercalciferol on the Factors Involved in Mineral Homeostasis
Figure 4
Effects of paricalcitol and doxercalciferol on serum PTH and PTH mRNA levels in uremic rats. The 5/6 NX rats on a hyperphosphatemia-inducing diet were treated with vehicle, paricalcitol, or doxercalciferol at 0.33 g/kg, i.p., 3 times/week, for two weeks ( = 8–11 per group). Blood samples and parathyroid gland were collected for the measurement of serum PTH (a) and PTH mRNA (b). For (b), real-time RT-PCR was performed as described in Materials and Methods. The mRNA expression level was first normalized to the GAPDH mRNA level and then calculated as % of control (Sham-vehicle, 100%). One way ANOVA Dunnett test with 95% confidence intervals of difference was performed for statistical comparisons. , versus Sham. , versus 5/6 NX-Vehicle.
