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International Journal of Endocrinology
Volume 2012 (2012), Article ID 381824, 15 pages
http://dx.doi.org/10.1155/2012/381824
Research Article

A Cell Model for Conditional Profiling of Androgen-Receptor-Interacting Proteins

Department of Paediatrics, Addenbrooke’s Hospital, University of Cambridge, Level 8, Box 116, Hills Road, Cambridge CB2 0QQ, UK

Received 7 September 2011; Revised 2 November 2011; Accepted 7 November 2011

Academic Editor: Olaf Hiort

Copyright © 2012 K. A. Mooslehner et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Partial androgen insensitivity syndrome (PAIS) is associated with impaired male genital development and can be transmitted through mutations in the androgen receptor (AR). The aim of this study is to develop a cell model suitable for studying the impact AR mutations might have on AR interacting proteins. For this purpose, male genital development relevant mouse cell lines were genetically modified to express a tagged version of wild-type AR, allowing copurification of multiprotein complexes under native conditions followed by mass spectrometry. We report 57 known wild-type AR-interacting proteins identified in cells grown under proliferating and 65 under nonproliferating conditions. Of those, 47 were common to both samples suggesting different AR protein complex components in proliferating and proliferation-inhibited cells from the mouse proximal caput epididymus. These preliminary results now allow future studies to focus on replacing wild-type AR with mutant AR to uncover differences in protein interactions caused by AR mutations involved in PAIS.