Research Article

A Cell Model for Conditional Profiling of Androgen-Receptor-Interacting Proteins

Table 1

List of only the known androgen-receptor-interacting proteins and coregulators (http://androgendb.mcgill.ca) identified after mass spec analysis of all gel fractions (Figure 3(a)) from FLAG purifications of nuclear extracts from P17 cells grown under proliferating and nonproliferating conditions (see Section 2). (a) Listed are the interacting proteins that were unique for the proliferating cells (33 only), interacting proteins that were unique for the nonproliferating cells (37 only), and (b) interacting proteins common to both (common). The AR is highlighted. Given are the Uniprot accession numbers identified by the database Mascot search (Uniprot), the score achieved in the peptide summary report (score), the gel slice number (), and information whether the protein had been identified as a coactivator (CoA), corepressor (coR), or the effect has not been reported (—). Activating function has been allocated to GSN [13, 14], without distinguishing between the 2 isoforms [15].
(a)

33 onlycoA/coRScore: P17/PC1 (slice)Uniprot37 onlycoA/coRScore: P17 (slice)Uniprot

KDM3ACoA 52 (3)Q6PCM1KDM5BcoA 93 (5)Q80Y84
WHSC1CoA 252 (5)Q8BVE8DAPK3coA 67 (1)O54784
MED17CoA 66 (2)Q8VCD5PIAS1coA/CoR 38 (2)O88907
MED24CoA 156 (3)A6PW47MED1coA 44 (6)Q925J9
ARID1BCoA 38 (6)E9Q4N7GATA3coA 55 (1)P23772
SRC 57 (1)Q2M4I4SP1coA 81 (3)O89090
SMARCC1CoA1112/60 (5)Q3UNN4TGFBI1coA 32 (1)Q62219
SMARCD1CoA 39 (1)Q61466TRIM24coA 165 (4)Q64127
NPM1CoA 438 (6)Q5SQB0FKBP5coA 29 (1)Q64378
GTF2F1 89 (2)Q3THK3RANBP10coA 53 (2)Q6VN19
CALCOCO1coA 156 (3)Q8CGU1
PSPC1coA 35 (2)Q8R326
GAKcoA 397 (5)Q99KY4
NCOA2coA 68 (5)Q61026
NR3C1coR 97 (3)Q06VW2
AP-1coA/CoR 56 (2)Q3TXG4
RB1coA 55 (5)Q3URY9
NF1CoA 46 (7)Q04690-1

(b)

CommoncoA/coRScore 33°C: P17/PC1 (slice)UniprotScore 37°C: P17 (slice)

STAT3CoA 41 (3)P42227 90 (3)
DAXXCoR 66 (4)Q3UIV3 70 (4)
ARID1ACoA 107 (5)A2BH40 78 (5)
HDAC1 CoR 428/52 (2)O09106 251 (2)
BRD7CoR 169 (3)O88665 50 (3)
HSP90CoR 719/250 (3)P07901 1089 (3)
GSN isoform1 [15]CoA ? 542 (4)P13020-1 516 (4)
GSN isoform2 [15]CoA ?1879/624 (3)P13020-2 1688 (3)
CALRCoR 148 (1)P14211 612 (1)
HSPA1BCoA 434 (2)P17879 582 (2)
ARCoA 115 (4)P19091200 (4)
XRCC6CoA1208/85 (2)P23475 1380 (2)
MCM3CoA 728 (3)P25206 1476 (3)
XRCC5CoA1727/49 (3)P27641 1717 (3)
PA2G4CoR 89/257 (1)P50580 54 (1)
ACTN4CoA/CoR3147/3229 (3)P57780 3112 (3)
ACTBCoA1386 (1)P60710 1106 (1)
DNAJA1CoR 173 (1)P63037 371 (1)
PRKDCCoA1134 (7)P97313 739 (7)
CTNNB1CoA 242/44 (3)Q02248 242 (3)
SMARCA4 CoA1841/73 (6)Q3TKT4 1449 (6)
MYST2CoR 437 (2)Q5SVQ0 36 (2)
KHDRBS1CoR 137/100 (2)Q60749 274 (2)
DDX5CoA1099/753 (2)Q61656 1150 (2)
SMARCA2CoA 727 (6)Q6DIC0 789 (6)
PPP2R1ACoR 433 (1)Q76MZ3 97 (1)
RBM14 232/201 (2)Q8C2Q3 373 (2)
COBRA1CoR 50 (6)Q8C4Y3 40 (6)
ATAD2CoA 405 (5)Q8CDM1 2221 (5)
KIAA1967CoA 39 (4)Q8VDP4 92 (4)
SFPQCoA/CoR 766/446 (3)Q8VIJ6 490 (3)
PRPF6CoA 259 (3)Q91YR7 218 (3)
NONOCoA/CoR 625/197 (1)Q99K48 472 (1)
PELP1CoA 261 (5)Q9DBD5 504 (5)
SART3CoR 286 (4)Q9JLI8 863 (4)
NCOR2CoR 107 (7)Q9WU42 95 (7)
PSMC3CoA 148 (1)A2AGN7 247 (1)
EHMT2CoA 66 (5)A2CG76 282 (5)
KDM1ACoA 210 (4)A3KG93 100 (4)
ZFP318CoR 35 (3)B0V2M3 30 (3)
EP300CoA 31 (7)B2RWS6 107 (7)
FLNACoA/CoR6598/1307 (7)B7FAU9 6972 (7)
BRD8CoA 62 (5)Q8R3B7 164 (5)
SUPERVILLINCoA 53/44 (6)Q8K4L3 439 (6)
DDX17 584/336 (2)Q3U741 645 (2)
HDAC6CoA 99 (4)Q3UG37 142 (4)
TRP53CoA1026/71 (1)Q80ZA1 1090 (1)
SRCAPCoA 57 (7)Q8BKT0 82 (7)