Distribution of Urocortins and Corticotropin-Releasing Factor Receptors in the Cardiovascular System
Figure 3
Immunocytochemistry of urocortin 3 in human heart and kidney. (a) and (b) Myocardium (Mc) was positively stained for uroccortin 3, whereas endocardium (Endo) and pericardial adipocytes (Adipo) were not. (c)–(e) Renal tubular cells in the renal cortex, particularly distal tubules, were strongly stained for urocortin 3 (shown by arrows in (d)); (d) a higher magnification of (c). (f) Renal tubular cells in the renal medulla were weakly stained for urocortin 3 (arrows). Typical urocortin 3-positive renal tubules were indicated by arrows (d)–(f). (g) and (h) Negative controls of kidney and heart using normal rabbit serum (1 : 1000). (g) A serial section of kidney (c); (h) a serial section of heart (b). Bars, 100 μm (reproduced from [30] with kind permission from the Endocrine Society, Copyright 2004, The Endocrine Society).
