Preventive Treatment with Methylprednisolone Paradoxically Exacerbates Experimental Autoimmune Encephalomyelitis
Figure 4
Homeostatic proliferation of transferred T cells after MP administration. C57Bl/6 mice were immunized with peptide and treated with 100 mg/kg MP, 100 mg/kg Dex, or PBS as a control on days −1, 0, and 1. One day after the last treatment, 1 × 107 purified CFSE-labeled T cells from RFP expressing mice were adoptively transferred, and ten days later, spleens and lymph nodes were analyzed by flow cytometry. (a) Representative histograms of lymph node total RFP+ T cells from PBS-(left), MP-(middle) or Dex-(right) treated mice are shown. (b) A quantification of the percentages of proliferating transferred T cells in the inguinal lymph nodes is depicted. for each group. Statistical analysis was performed using the unpaired -test (**, ***).
