Thyroid Peroxidase Gene Mutation in Patients with Congenital Hypothyroidism in Isfahan, Iran

Table 1

Primers used for PCR Amplification of thyroid peroxidase gene.

Exon	Forward Primers		Reverse Primers		Fragment size (bp)	Annealing temperature
	Position of 5^′ end	Nucleotide sequence (5^′→3^′)	Position of 5^′ end	Nucleotide sequence (5^′→3^′)
(1)	−70	GACTTCCTAGCATCTTGACG	+67	CACTTTACAAGTTCCAATGATG	220	58^°C
(2)	−74	AGACAAGGACACAGCGGTTC	+95	CATGGCCTTGTCAGTGCTTG	225	60^°C
(3)	−67	AAGCAACACTGTCAGTGAATC	+123	TTAACAATGGCAAGCTTCAG	275	60^°C
(4)	−60	TT AAGTACCAAAGATACCATAGAC	+65	CACAAAGTCAAGGTGTCCTC	295	60^°C
(5)	−101	CAAATTCAGATGCTGGAGTCAC	+73	TCCTTCATGATGGCATCTAGTC	308	61^°C
(6)	−86	CTGAGAATGGTGTCTTATATCTG	+52	AGCATCACAGGACCCAATC	313	61^°C
(7)	−61	GTCATCTTTCTGCTACCACG	+60	TTGACGTTTTAAATAGCACTTAG	327	55^°C
(8)	−60	AGAGTCTTACAA AGG GTG CAC	+163	AAG TAC CTG GGA GAG AGA AGC	678	60^°C
(9)	−29	TCA CTGAGATGCTTTTCCTAT C	+45	AAGAGTTCATGGGGACCAG	327	60^°C
(10)	−55	GTTTCTCTAGAACTGAGCCAAG	+79	AGTCTCTCTAGCAGCAGGTTG	306	61^°C
(11)	−51	AACAAAAGTTCAGTTCTGTGAGAG	+44	TGTGCAGAACGTGAAGGAAG	330	61^°C
(12)	−42	CTC CAT GCA CTG TGA CCT TAC	+57	CTTTGTTTGATGAGATGCACG	308	61^°C
(13)	−46	CTTTTCTCGTAGTTTGACTACATG	+54	CTTATATCGGAAACATTCAGATG	271	60^°C
(14)	−69	AGAGAAGCACCTCCCAGAAC	+69	TACAAAAACTCGCAAATGGAC	270	61^°C
(15)	−75	CAGACTCAGGCAGGACAACC	+69	ATTGCAGCCATGTCCAGAG	244	61^°C
(16)	−61	CTACCCTCCACAGTCACGGT	+59	CCAGATCCTGTCCAACCACT	250	62^°C
(17)	−108	TGTGAAAAGAGCTCCTGTC	+49	GTGATTTTGGGAACATGAAG	211	62^°C