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International Journal of Endocrinology
Volume 2012 (2012), Article ID 947187, 10 pages
http://dx.doi.org/10.1155/2012/947187
Research Article

Nerium oleander Distillate Improves Fat and Glucose Metabolism in High-Fat Diet-Fed Streptozotocin-Induced Diabetic Rats

1Pharmacology and Toxicology Department, Faculty of Veterinary Medicine, Selcuk University, 42070 Konya, Turkey
2Physiology Department, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, 15030 Burdur, Turkey
3Pathophysiology Department, Faculty of Medicine, Ankara University, 06100 Ankara, Turkey
4Molecular Biology Research and Development Unit, Faculty of Medicine, Ankara University, 06100 Ankara, Turkey

Received 31 July 2012; Revised 4 October 2012; Accepted 22 October 2012

Academic Editor: Daniela Jezova

Copyright © 2012 Ahmet Levent Bas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Diabetes was induced by intraperitoneal injection of streptozotocin (35 mg/kg bw) in all rats of five groups after being fed for 2 weeks high-fat diet. Type 2 diabetic Nerium-oleander- (NO-) administered groups received the NO distillate at a dose of 3.75, 37.5, and 375 μg/0.5 mL of distilled water (NO-0.1, NO-1, NO-10, resp.); positive control group had 0.6 mg glibenclamide/kg bw/d by gavage daily for 12 weeks. Type 2 diabetic negative control group had no treatment. NO distillate administration reduced fasting blood glucose, HbA1c, insulin resistance, total cholesterol, low density lipoprotein, atherogenic index, triglyceride-HDL ratio, insulin, and leptin levels. Improved beta cell function and HDL concentration were observed by NO usage. HDL percentage in total cholesterol of all NO groups was similar to healthy control. NO-10 distillate enhanced mRNA expressions of peroxisome proliferator-activated-receptor- (PPAR-) α, β, and γ in adipose tissue and PPAR-αγ in liver. The findings from both in vivo and in vitro studies suggest that the considerable beneficial effect of NO distillate administration at a dose of 375 μg/0.5 mL of distilled water may offer new approaches to treatment strategies that target both fat and glucose metabolism in type 2 diabetes.