948683.fig.004a
(a)
948683.fig.004b
(b)
948683.fig.004c
(c)
948683.fig.004d
(d)
Figure 4: The characteristics of our new culture system. (a) The photo of pancreatic cells cultured in inverted dish for 1 h and 24 h. (b) The morphology of spheres cultured for 1 and 7 days at the air-medium interface. (c) Immunohistological analysis of insulin, glucagon, and CPA staining in spheres cultured for 7 days. (d) The expressional dynamics of Ngn3 and insulin mRNA in spheres cultured for 1, 4, 7 days.