Isolation of Pancreatic Progenitor Cells with the Surface Marker of Hematopoietic Stem Cells
Figure 4
The characteristics of our new culture system. (a) The photo of pancreatic cells cultured in inverted dish for 1 h and 24 h. (b) The morphology of spheres cultured for 1 and 7 days at the air-medium interface. (c) Immunohistological analysis of insulin, glucagon, and CPA staining in spheres cultured for 7 days. (d) The expressional dynamics of Ngn3 and insulin mRNA in spheres cultured for 1, 4, 7 days.