125932.fig.002a
(a)
125932.fig.002b
(b)
125932.fig.002c
(c)
125932.fig.002d
(d)
Figure 2: Recombinant PCI-hOPGp-mOPG plasmid construction and plasmid linearization. (a) mCPG DNA was digested by Not I and Sal I. (b) PCI-NEO-OPG-LacZ was digested by restriction enzymes Not I and Sal. (c) Identification of the digested pCI-hOPG-mOPG.M:Marker1Kb Ladder. 1: pCI-hOPG-mOPG was digested by NotI and SalI; 2: pCI-hOPG-mOPG was digested by EcoRI; 3: pCI-hOPG-mOPG plasmid. (d) linear pCI-hOPGp-mOPG plasmid DNA.M:1KbLadder; 1: pCI-hOPG-mOPG palsmid; 2: pCI-hOPG-mOPG digested by BglII/KpnI; 3: pCI-hOPG-mOPG digested by BglII/KpnI was recovered.