Photoperiod-Dependent Effects of 4-tert-Octylphenol on Adherens and Gap Junction Proteins in Bank Vole Seminiferous Tubules

Figure 5

The effects of ICI 182,780 on octylphenol-induced decrease of N-cadherin (a), β-catenin (b) and connexin 43 (c) protein expression in homogenates of testis explants from bank voles kept in long light cycles (𝑛=8). Explants were cultured in control medium (c) or in media containing 6 mg/L ICI 182,780 (ICI); 100 or 500 mg/mL 4-tert-octylphenol (100, 500); 100 or 500 mg/mL 4-tert-octylphenol and 6 mg/L ICI 182,780 (100+ICI; 500+ICI) for 24 h. Graphs represent the relative amount of N-cadherin (b), β-catenin (c), and connexin 43 (d) protein normalized to β-actin. Data obtained from three separate analyses is expressed as mean ± SD. Significant differences from control values are denoted as ^∗𝑃<0.05 and ^∗∗𝑃<0.01.

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