Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 Pathway

Figure 6

Effects of atorvastatin on mRNA and protein expression of HMG-CoAR, LDLr, SREBP-2, SCAP, and nSREBP-2. mRNA levels were determined by real-time RT-PCR as described in Section 2. GAPDH was used as a reference gene. Data are means ± SD (a). Protein levels were examined by western blotting (b). Histogram represents means ± SD of the densitometric scans of HMG-CoAR, LDLr, SREBP-2, and SCAP proteins, normalized by comparing with actin and expressed as a percentage of control (c). Protein level and histogram of nSREBP-2. Data are means ± SD ((d) and (e)). Atorvastatin treatment increased the mRNA and protein expression of HMG-CoAR, LDLr, SREBP-2, and nSREBP-2, but no change was observed in SCAP. *𝑃<0.05 versus DM; ^#𝑃>0.05 versus DM.

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