679763.fig.003a
(a)
679763.fig.003b
(b)
679763.fig.003c
(c)
679763.fig.003d
(d)
679763.fig.003e
(e)
679763.fig.003f
(f)
Figure 3: Cell apoptosis analysis in C20/A4 cells transfected with mutant Wisp3 Cells stably transfected with /pcDNA3.1(+), /pcDNA3.1(+), or empty vector; cell apoptosis was evaluated by using FAC flow cytometry (a–c) and acridine orange/ethidium bromide staining (d–f) (apoptotic cells stained with yellow, condensed, or fragmented nuclei) analysis. (a) and (d) empty vector apoptosis rate is 27.1%; (b and e) apoptosis rate is 9.2%; (c and f) apoptosis rate is 7.8%. Magnification is 200 x.