Dysfunction of Collagen Synthesis and Secretion in Chondrocytes Induced by Wisp3 Mutation

Figure 3

Cell apoptosis analysis in C20/A4 cells transfected with mutant Wisp3 Cells stably transfected with MUT^1000T/C/pcDNA3.1(+), MUT^840delT/pcDNA3.1(+), or empty vector; cell apoptosis was evaluated by using FAC flow cytometry (a–c) and acridine orange/ethidium bromide staining (d–f) (apoptotic cells stained with yellow, condensed, or fragmented nuclei) analysis. (a) and (d) empty vector apoptosis rate is 27.1%; (b and e) MUT^1000T/C apoptosis rate is 9.2%; (c and f) MUT^840delT apoptosis rate is 7.8%. Magnification is 200 x.

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