Dysfunction of Collagen Synthesis and Secretion in Chondrocytes Induced by Wisp3 Mutation
Figure 4
mRNA expression of cartilage-specific genes in C20/A4 cells transfected with wild and mutant Wisp3. Cells stably transfected with WT-Wisp3/pcDNA3.1(+), /pcDNA3.1(+), /pcDNA3.1(+), or empty vector. mRNA expression of cartilage-specific genes in C20/A4 cells was determined by RT-PCR. (a)–(g) represent Wisp3, COL2A1, COL1A1, SOX9, fibronectin, MMP-1, and β-actin separately.