Dysfunction of Collagen Synthesis and Secretion in Chondrocytes Induced by Wisp3 Mutation

Table 1

Primer pairs and experimental conditions.

Gene product	Forward and reverse primers (5′-3′)	Expected product size, bp	Annealing temperature (°C)
COL2A1	CCTAATGGAGATGCTGGTCG	187 bp	57
	CCAGGGAATCCAATGTTGC
COL1A1	ATCCAGCTGACCTTCCTGCG	322 bp	60
	TCGAAGCCGAATTCCTGGTCT
Wisp3	GTAAGCTTAGCGACATGCAGGGGCTCCTCTT	1065 bp	62
	GCGGATCCTTACAGAATCTTGAGCTCAG
Fibronectin	GTGTGACCCTCATGAGGCAAC	299 bp	60
	TACTCTCGGGAATCTTCTCTGT
SOX9	CACACTACAGCCCCTCCTAC	258 bp	60
	CCTCCTCAAGGTCGAGTGAG
MMP-1	ATGCTGAAACCCTGAAGGTG	305 bp	60
	CAAGATTTCCTCCAGGTCCA
𝛽-actin	TCCTGTGCATCCACGAAACT	310 bp	58
	GAAGCATTTGCGGTGGACGAT