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Figure 3: Effects of puerarin on the expression of Bcl-2 and Bax in hOBs. Cells were treated with 0, 10−9 M, 10−8 M, and 10−7 M puerarin for 48 h before collecting proteins. Cell lysates were subjected to western blot analysis and incubated with anti-Bax, anti-Bcl-2, or anti-β-actin monoclonal antibodies. Gel-Pro 4.0 gel image analysis software was used to analyze the absorbance values of Bcl-2 and Bax.