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International Journal of Endocrinology
Volume 2013 (2013), Article ID 858206, 5 pages
http://dx.doi.org/10.1155/2013/858206
Research Article

Determinants of Microbial Load in Infected Diabetic Foot Ulcers: A Pilot Study

1Outpatient Clinic of the Diabetic Foot, Second Department of Internal Medicine, Democritus University of Thrace, University Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, Greece
2Microbiology Laboratory, Democritus University of Thrace, University Hospital of Alexandroupolis, Dragana, 68100 Alexandroupolis, Greece

Received 29 May 2013; Accepted 10 June 2013

Academic Editor: Ilias Migdalis

Copyright © 2013 M. Demetriou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We examined the determinants of microbial load in infected diabetic foot ulcers in 62 patients (38 men and 24 women, mean age: years) with clinically infected diabetic foot ulcers. Tissue cultures were taken from ulcers by 4 mm punches. Ulcer grade (University of Texas classification), neuropathy disability score (NDS), neuropathy symptom score (NSS), ankle-brachial index (ABI), perfusion, extent, depth, infection, and sensation (PEDIS) grade of diabetic foot infection, and laboratory parameters were evaluated in all patients. Total microbial load was positively correlated with the number of isolates on tissue cultures ( , ), white blood cell count (WBC) ( , ), and platelet count (PLT) ( , ). It also exhibited a borderline insignificant positive correlation with PEDIS infection grade ( , ). In stepwise linear regression analysis, the number of isolates on tissue cultures and WBC were identified as the only two significant parameters accounting for 38% of the variation in the log of total microbial load (adjusted , ). In conclusion, patients with infected diabetic foot ulcer exhibit a positive correlation of total microbial load with the number of isolates on tissue cultures, WBC and PLT.