Endocannabinoids and Endovanilloids: A Possible Balance in the Regulation of the Testicular GnRH Signalling
Figure 3
Effects of AEA treatment on GnRH-1 (a) and GnRH-2 (c) expression in frog testis collected from June animals (/group) after 1 h of incubation. Incubations have been carried out with AEA 10−9 M, SR 10−8 M, or both. C0: untreated testis of June; C: control group, testis treated with Krebs-Ringer buffer. Effects of cap treatment on GnRH-1 (b) and GnRH-2 (d) expressions in frog testis of June after 1 h of incubation. Incubations have been carried out with cap 10−6 M, cpz 10−5 M, SR 10−8 M, or combinations of cap/cpz and SR/cpz. C0: untreated testis of June; C1: control group, testis treated with Krebs-Ringer buffer. The data in graph are the results of RT-PCR analysis; they are reported as fold increase (FI) calculated comparing the expression of GnRH-1/GnRH-2 to the housekeeping fp1 and are representative of three separate experiments at least (). Asterisks indicate statistically significant differences.
