Endocannabinoids and Endovanilloids: A Possible Balance in the Regulation of the Testicular GnRH Signalling
Figure 5
Effects of AEA treatment on cb1 (a) expression in frog testis collected from June animals (/group) after 1 h of incubation. Incubations have been carried out with AEA 10−9 M, SR 10−8 M, or both. C0: untreated testis of June; C: control group, testis treated with Krebs-Ringer buffer. Effects of cap treatment on cb1 (b) expression in frog testis of June after 1 h of incubation. Incubations have been carried out with cap 10−6 M, cpz 10−5 M, SR 10−8 M, or combinations of cap/cpz and SR/cpz. C0: untreated testis of June; C1: control group, testis treated with Krebs-Ringer buffer. The data in graph are the results of RT-PCR analysis; they are reported as fold increase (FI) calculated comparing the expression of cb1 to the housekeeping fp1 and are representative of three separate experiments at least (). Asterisks indicate statistically significant differences.