Table 2: Primers used for real-time RT-PCR.

Target geneSequenceLength (bp)

MAFbx/atrogin-1S
AS
5′-GGCGGACGGCTGGAA-3′  
5′-CAGATTCTCCTTACTGTATACCTCCTTGT-3′
100
MuRF-1S
AS
5′-ACGAGAAGAAGAGCGAGCTG-3′  
5′-CTTGGCACTTGAGAGAGGAAGG-3′
179
GAPDHS
AS
5′-CGTGTTCCTACCCCCAATGT-3′  
5′-ATGTCATCATACTTGGCAGGTTTCT-3′
74

AS: antisense primer; S: sense primer; MAFbx/atrogin-1: muscle atrophy F-box protein; MuRF-1: muscle-specific RING finger protein 1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.