Major Histocompatibility Class II Pathway Is Not Required for the Development of Nonalcoholic Fatty Liver Disease in Mice
Figure 1
MHCII gene genotyping and hepatic expression in wild-type and MHCIIΔ/Δ mice. (a) Two different PCRs were performed for the detection of wild-type (WT PCR) and knockout (KO PCR: MHCIIΔ/Δ) fragments; 173 bp for a WT band and 209 bp for a KO band. Therefore heterozygous (Het) presents both bands. (b) Gene expression of MHC II genes (H2-Eb1, -Aa, -Ab1) in liver samples by real-time RT-PCR. The expression was normalized by β2 microglobulin and compared to the wild-type. Open bars represent wild-type and closed bars MHCIIΔ/Δ mice ( = 10–14/group). Data are presented as mean ± SEM. *Significantly different from wild-type mice, (Student t-test).
